The genomes were from infected patients in 68 countries. We identified variants by extracting pairwise alignment to the reference genome NC_045512, using the EMBOSS needle. Nucleotide variants in the coding regions were converted to corresponding encoded amino acid residues The bioinformatics analysis showed 44 different nucleotide mutations that caused 26 nonsynonymous mutations in protein sequences with regard to the reference full genome of the SARS-CoV-2 sequence (NC_045512.2). R207C, V378I, M2796I, L3606F, and A6407V in ORF1ab were common mutations in these sequences. Also, some of the detected mutations only were found in Iranian data in comparison with all. .2 identisch werden ausschließlich übereinstimmende alignierte informative Positionen (A, T, G, C) gewertet. Nummer 3: Positionen im rekonstruierten Genom, die von weniger als 20 abgedeckt sind, müssen mit N . Reads maskiert werden. Sofern PCR Duplikate entfernt werden können, kann die kale Sequenziertiefeminimale lo. au
.2 / MN908947.3 YP_009724390.2 / QHD43423.2. GeneID: 43740575. Human ACE2 Receptor Protein. Accession: NM_021804.3 NP_068576.1. GeneID: 59272. Coronavirus. Coronaviruses (CoVs) are a large group of viruses that commonly originate in many different species of animals. Some coronaviruses can make a jump from animals to human, and cause the respiratory infections including mild and cold. Sarbecovirus ist eine Untergattung innerhalb der Gattung Betacoronavirus in der Unterfamilie Orthocoronavirinae und der Familie Coronaviridae. Sie enthält bisher nur eine Spezies. Diese trägt den Namen Severe acute respiratory syndrome-related coronavirus , oder kurz SARS-related coronavirus Search, retrieve, and analyze sequences and other content in the NCBI Virus SARS-CoV-2 Data Hub Interactive Dashboard. Download viral genome and protein sequences, annotation, and a data report from NCBI Datasets. Get the latest list of SARS-CoV-2 nucleotide sequences. You can query these IDs in GenBank
SARS-CoV-2 proteins - NCBI Datasets. Select and download a protein dataset from complete SARS-CoV-2 genomes, including nucleotide and protein sequences, annotation, protein structures and a detailed data report. 0 complete annotated genomes match these criteria. RefSeq and GenBank. RefSeq only SARS-CoV-2 Results. ScanFold results for the COVID-19 strain (SARS-CoV-2; NC_045512.2) can now be found on the RNAStructuromeDB: browse the results in IGV below, on JBrowse or download all ScanFold output files here. The SARS-CoV-2 genome is a single stranded RNA molecule approximately 30,000 nucleotides long. The ScanFold program has been used. UCSC Genome Browser on SARS-CoV-2 Jan. 2020/NC_045512.2 Assembly (wuhCor1) move zoom in zoom ou entwickelt: NC_045512.2 (National Center for Biotechnology Information (NCBI)). Die untere Nachweisgrenze (Limit of Detection, LoD) wurde mit quantiﬁ zierter SARS-CoV-2-speziﬁ scher RNA (In-vitro-Transkripte (IVT)) bestimmt. Die Nachweisgrenze wurde in drei unabhängigen Untersuchungen mit drei unabhängigen Chargen mit jeweils 21 Replikaten in Anwesenheit von 200 ng huma- ner.
number NC_045512) from 16 to 29826 (29810/29811), except at site 24019, with the same substitution of a C from isolate Wuhan-Hu-1 for T. The C24019T mutation corresponds to C24034T if we use the sequence located under GISAID strain identiﬁer EPI_ISL_405839 as a reference. This was a silent mutation at the spike gene (codon AAC to AAT). Based on the reference sequence, the following ﬁve. The Illumina sequences were combined combined, and a reference-guided assembly was performed against the complete genome sequence for SARS-CoV-2 strain Wuhan-Hu-1 (GenBank accession number NC_045512.2). For data from laboratory A, amplification primers and low-quality bases were trimmed from mapped reads using iVar v. 1.1 with default settings (slide window size, 4 bp; minimum quality score. Reference Sequence: NC_045512). Nucleic acid amplification technology (NAAT) based on poly-merase chain reaction (PCR), is regarded as the gold standard for virus detection. The diagnosis of COVID-19 relies on the detection of the SARS-CoV-2 RNA by RT-PCR.3, 4 The viral RNA is detectable in nasopharyngeal and oropharyngeal swab samples as well as respiratory secretions during the acute. The Basic Local Alignment Search Tool (BLAST) finds regions of local similarity between sequences. The program compares nucleotide or protein sequences to sequence databases and calculates the statistical significance of matches. BLAST can be used to infer functional and evolutionary relationships between sequences as well as help identify members of gene families The sequence of the genome is available here: NC_045512.2 version of the SARS-CoV-2 viral genome. We'll synthesize: the DNA encoding all of these SARS-CoV-2 proteins on plasmids for expression in E. coli and S. cerevisiae, along with pull down tags (e.g. 6xHis) and T7 promoter for mRNA production. Viral protein sequences with the addition of.
NC_045512.2 28.0 17.6 24.0 No rank 2697049 SARS-CoV-2 COG-UK 38 124 NC_045512.2 28.0 25.5 58.0 No rank 2697049 SARS-CoV-2 NMDC 295 NC_045512.2 28.1 28.8 56.5 Species 694009 SARS-CoV GenBank + RefSeq 673 NC_004718.3 14.0 91.6 19.7 Species 1335626 MERS-CoV GenBank + RefSeq 1381 NC_019843.3 27.0 104.0 87. Das Virus SARS-CoV-2 (Abk. für englisch severe acute respiratory syndrome coronavirus type 2 ), auch als Schweres akutes Atemwegssyndrom -Coronavirus-Typ 2 bezeichnet, umgangssprachlich (neuartiges) Coronavirus genannt, ist ein dem SARS-Erreger ähnliches Betacoronavirus mit wahrscheinlich zoonotischem Ursprung TAYLOR (Trimming Amplicons You LOve Rapidly) This pipeline takes gzipped fastq files and outputs .bam files aligned to NC_045512.2 as well as consensus fastas. Notably this pipeline incorporates primerclip. By default input files should be paired-end fastq files to cover the 116-255 bp amplicons produced from the Swift Amplicon SARS-CoV-2 Panel NCBI Genes from NC_045512.2 (All Genes and Gene Predictions tracks) Display mode: Label: Alternative/human readable gene name Primary identifier for gene NCBI Gene ID Protein Product . Color track by codons: Help on codon coloring Show codon numbering: Display data as a density graph:. (NC_045512.2) and two partial sequences from Italian isolates (EPI_ISL_406959 and EPI_ISL_406960). To compare 2019 ‐nCoVs with closely related viral species, we obtained six sequences from distinct human SARS genomes from GenBank (the reference NC_004718.3, plus the genomes AY274119.3, GU553363.1, DQ182595.1, AY297028.1, and AY515512.1). We also obtained six BCoV genomic sequences (DQ022305.
Wuhan NC_045512.2 (GenBank reference genome for 2019‐nCoV) BCoV bat‐SL‐CoVZXC21 MG772934.1 (Bat virus similar to 2019‐nCoV) Bat SARS coronavirus HKU3‐1 DQ022305.2 (Bat virus more distantly related to 2019‐nCoV) SARS NC_004718.3 (GenBank reference genome for SARS) Our analysis shows a close homology for all proteins with Bat sequence MG772934.1 (>80%), and more distant with the. quence (GenBank accession no. RefSeq NC_045512), trimmed primers based on position, and called vari-ants with Medaka (https://github.com) (Appendix). Each Medaka variant was filtered by coverage depth (>20×) and by the Medaka model-derived variant quality (>30). We used the variant quality score as a heuristic to filter remaining noise from the Medaka variants compared with Sanger-derived. Plays a central role in virus morphogenesis and assembly. Acts as a viroporin and self-assembles in host membranes forming pentameric protein-lipid pores that allow ion transport. Also plays a role in the induction of apoptosis (By similarity). May interfere with tight-junction stability by interacting with host MPP5. This would result in disruption of epithelial barriers, thereby amplifying.
On February 5, mNGS reported 3,460 sequences that showed 99.8% identity and covered 98.69% of the SARS-CoV-2 genome NC_045512.2|SARS-CoV-2|Wuhan-Hu-1 (GenBank accession no. NC_045512.2). We then performed rRT-PCR by using newly collected sputum and stored BALF, which also tested positive. Cycle threshold values were 34 for sputum and 30 for BALF. However, a fourth nasopharyngeal swab collected. Gen Nc_045512-S Glycoprotein de SARS-CoV-2_isolate Wuhan-Hu-1_jp2.zip (View Contents) 20-Nov-2020 19:37: 1.7M: Gen Nc_045512-S Glycoprotein de SARS-CoV-2_isolate Wuhan-Hu-1_page_numbers.json: 20-Nov-2020 20:03: 602.0B: Gen Nc_045512-S Glycoprotein de SARS-CoV-2_isolate Wuhan-Hu-1_scandata.xml: 20-Nov-2020 20:03: 1.1K __ia_thumb.jpg: 15-Feb-2021. The reference NC_045512.2 SARS-CoV-2 Wuhan genome (Coronaviridae Study Group of the International Committee on Taxonomy of Viruses, 2020), 29,903 nucleotides long, was obtained from NCBI GenBank. A GFF3 annotation associated to the refence, showing genomic coordinates for all protein sequences of SARS-CoV-2, is provided as Supplementary File 3 Libraries were constructed using the QIAseqFX kit or the NEBNext ARTIC SARS-CoV-2 FS Library Prep Kit (Illumina) and sequenced on a MiSeq instrument (2x75 bp). Coverage depth per base was determined, reads were down-sampled with seqtk and aligned to SARS-CoV-2 reference genome (NCBI, NC_045512) with Bowtie2 SARS-CoV-2. We have released a new version of V-pipe specifically adapted to analyze high-throughput sequencing data of SARS-CoV-2. available in the sars-cov2 branch of the Github repository. default reference sequence is NC_045512, identical to GenBank entry MN908947. default configuration file assumes SARS-CoV-2 NGS data
4. Extraction of reads mapped exclusively to NC_045512.2. 5. Calculation of coverage and hybrid selection metrics across NC_045512.2 and GrC38. 6. Calculation of the validity of the sample. If the. Consensus sequence was reconstructed and mapped to the SARS-CoV-2 reference sequence NC_045512.2 using Bowtie2 in sensitive-local mode with consensus threshold at 65% . The variant calling was carried out by the Variant Finder Tool (Geneious) filtering out variants with a p value greater than 0, using a minimum variant frequency of 0 and default parameters for Maximum Variant p-value (10 −6 ) Libraries were constructed using the NEBNext ARTIC SARS-CoV-2 Library Prep Kit (Illumina) and sequenced on a MiSeq® instrument (2x250 bp). Coverage depth per base was determined, reads were down-sampled with seqtk and aligned to SARS-CoV-2 reference genome (NCBI, NC_045512) with Bowtie2
2019-nCoV_E control: Severe acute respiratory syndrome coronavirus 2 isolate Wuhan-Hu-1, complete genome (GenBank: NC_045512.2) Don't see what you need for your nCoV experiments? We can accommodate custom requests. Contact us. References (2020) Detection of 2019 novel coronavirus (2019-nCoV) by real-time RT-PCR. [Online] Eurosurveillance. [Accessed 8 Apr 2020]. Resources. Safety data sheets. A complete genome sequence was obtained for a severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) strain isolated from an oropharyngeal swab specimen of a Nepalese patient with coronavirus disease 2019 (COVID-19), who had returned to Nepal after traveling to Wuhan, China To capture the full coding capacity of SARS-CoV-2, we applied a range of ribosome-profiling approaches to Vero E6 cells infected with SARS-CoV-2 for 5 or 24 h (Fig. 1a ). At 24 h post-infection.
Sequence Reference: NC_045512.2 (with D614G mutation) Codon optimized and R683/5A mutations; ORF size: 3765 bp Sequencing primers: - Forward HTLV 5'UTR: TGCTTGCTCAACTCTACGTC - Reverse SV40 pAn: AACTTGTTTATTGCAGCTT Quality Control: - Plasmid construct is confirmed by restriction analysis and full‑length open reading frame (ORF) sequencing. - After purification by ion-exchange chromatography. The World Health Organization (WHO) recognized OncoGen, as the only research centre in Romania that has a protein subunit COVID-19 vaccine candidate in pre-clinical tests. OncoGen joins reputable academic institutions and private companies known for their expertise in the field of vaccine development and high-performing biomedical research
We modelled the full SARS-CoV-2 proteome based on the NCBI reference sequence NC_045512 and annotations from UniProt. The results are available here. Variant of Concern 202012/01. We annotated the mutations of the newly emerging Variant of Concern that appeared in December 2020 in the UK. This annotation project shows the location of the mutations on the wild type proteins. What's new. We. We used the complete genome sequence SARS-CoV-2 Wuhan-Hu-1 strain (Accession NC_045512, Version NC_045512.2) as a reference genome. The annotated genomes (n = 2,492) have. NC_045512: Sequence Length: 29903: Sequence Status: Complete: Sequence: View Nucleotide Sequence and design PCR primers: Number of Proteins: 66: Organism Name: Severe acute respiratory syndrome coronavirus 2: Mol Type: genomic RNA: GenBank Host: Homo sapiens: Host: Human: Isolation Country: China: Collection Date: 2019-12: Genome Image Map. Hide Show Toggle Wrapping Scale: Protein Information.
The complete genome sequence of SARS-CoV-2 isolate Wuhan-Hu-1 (GenBank accession number NC_045512) was used as the reference genome for simulating mutations. The simuG program (Yue and Liti, 2019) was used to simulate random point mutations and indels to the reference genome sequence. The number of mutations in each simulated genome sequence was drawn from a Poisson distribution based on the. NC_045512.2: 28881: N: GGG>AAC: G: G: R203_G204delinsKR Figura 2. Evidențierea SNVs prin comparare cu tulpina de referință Severe acute respiratory syndrome coronavirus 2 isolate Wuhan-Hu-1 (NC_045512.2) Identificarea variantelor virale trebuie atent interpretată și monitorizată pentru întelegerea adaptării, evoluției și transmiterii virusului SARS-CoV-2 în populația umană, precum. Prepare libraries from input RNA using the robust KAPA RNA HyperPrep Kit and then enrich for viral sequences; this panel targets 100% of the reference SARS-CoV-2 genome (NC_045512) and >99.7% of another 183 publicly available SARS-CoV-2 genomes (GenBank). Identify multiple variants of SARS-CoV-2 in a single reactio
SARS-CoV-2 Coronavirus Data Compression Benchmark. Innar Liiv, Ph.D, IEEE Senior Member. Last update: 23 February 2021. CALL FOR PARTICIPANTS! Challenge: Compress the 1,317,937,667 bytes Coronavirus dataset to less than 613,466 bytes! It seems to me that the most important discovery since Gödel was the discovery by Chaitin, Solomonoff and. Die meisten Mutationen in unserem Isolat bestanden zu 70% aus alternativen Genen und zu 30% aus Referenzgenen (NC_045512). dies kein Beleg dafür wäre, dass man hier ebenfalls SARS-CoV-2 nachgewiesen habe. Klar, wenn die Grundlage bereits keinen Beweis darstellt, kann ein Vergleich zu dieser nicht automatisch als Beweis und Bestätigung der Grundlage dienen. Die Autoren dieser Studie haben. Full-length virus genomes were annotated using the reference genome of hCoV-19/Wuhan/Hu-1/2019 (NC_045512.2) and then visualized in UGENE v. 1.30. For phylogenetic analysis, a dataset of 88 available SARS-CoV-2 complete genomes from different countries, including Indonesia, was retrieved from GISAID. Results. All patients were hospitalized with various severities of COVID-19. Phylogenetic. EzCOVID19 is a cloud-based bioinformatics platform for rapid detection, identification and characterization of SARS-CoV-2 virus from raw metagenomic, metatranscriptomic, RNA-seq and/or isolate (amplicon or enrichment) next-gen sequence data suspected of containing the SARS-CoV-2 virus NC_045512.2. Page . 3. of . 9. 3. Two SARS-CoV-2 positive amplification curve controls (low and high) are included on each amplification plate to ensure that SARS-CoV-2 RNA can be detected by the.
An unresolved issue of SARS-CoV-2 disease is that patients often remain positive for viral RNA as detected by PCR many weeks after the initial infection in the absence of evidence for viral replication. We show here that SARS-CoV-2 RNA can be reverse-transcribed and integrated into the genome of the infected cell and be expressed as chimeric transcripts fusing viral with cellular sequences SARS-CoV-2 (Abk. für englisch severe acute respiratory syndrome coronavirus 2, deutsch Schweres-akutes-Atemwegssyndrom-Coronavirus 2, umgangssprachlich nur (neues) Coronavirus genannt; vormals auch 2019-nCoV, 2019-novel Corona virus , neuartiges Coronavirus 2019 sowie Wuhan-Coronavirus) ist ein im Dezember 2019 in der chinesischen Stadt Wuhan, Provinz Hubei, neu. The sequences of spike proteins of 2019-nCoV (Wuhan-HU-1, Accession NC_045512) and of SARS CoV (GZ02, Accession AY390556) were aligned using MultiAlin software. The sites of difference are.
Wuhan reference strain (NC_045512.2) using minimap version 2.17,112 and the SNPs, including 113 the corresponding amino acid changes, were called using the Geneious Prime SNP caller [14, 114 15]. Amino acids identical to the reference strain at the investigated loci were considered wild-115 type; else, they were considered mutants. Only. COVID-19, caused by the novel SARS-CoV-2 virus, started in China in late 2019, and soon became a global pandemic. With the help of thousands of viral genome sequences that have been accumulating, it has become possible to track the evolution of the viral genome over time as it spread across the world. An important question that still needs to be answered is whether any of the common mutations. The reference genome sequence of the SARS‐CoV‐2 of Wuhan (accession‐id: NC_045512) was included for phylogenetic analysis. All the genomes were aligned by the Multiple Alignments Fast Fourier transform algorithm and sequences were cleaned with Block Mapping and Gathering with Entropy 16 cleaning algorithm. The Mega X 17 desktop application was used to generate and visualize the. 64 NC_045512.2 for strain Wuhan -Hu -1, were prepared and used as real -time RT -PCR standards 65 for quantification of genome equivalent copy number (GE) of SARS -CoV -2 RNA . Real -time 66 RT -PCR was performed using the protocol from Carman et al. (15) with Super Script III one -step 67 RT -PCR System and Platinum Taq Polymerase . The QuantStudio 7 Flex Real -Time PCR System. CDC assays for SARS-CoV-2 detection. The US Centers for Disease Control and Prevention (CDC) has designed RT-PCR assays and published a protocol for the detection of the 2019-nCoV. IDT provides primers and probes for these CDC assays for the identification of virus. The primers and probes are manufactured at IDT under ISO 13485:2016 conditions.
These nucleotide sequences were aligned to the GenBank reference sequence (accession ID: NC_045512.2) and then translated into amino acid residues according to the coding sequence positions provided along the reference sequence for SARS-CoV-2 proteins (orf1a, orf1b, S, ORF3a, E, M, ORF6, ORF7a, ORF7b, ORF8, N, and ORF10). These sequences were aligned separately for each protein using the MAFFT. The MSA analysis of the CDS sequence coding for the partial RNA binding domain of N protein of SARS-CoV-2 isolated in Iran (MT186676) and the query CDS sequence from Wuhan, China (NC_045512.2) showed high similarity in both CDS sequences. A nucleotide mismatch was identified at nucleotide No. 312 that mutates T to C
Most of the mutations in our isolate consisted of 70% alternative genes and 30% reference genes (NC_045512). Five variants were found in ORF1ab, one variant in S gene, two variants in ORF3a, and one variant in E gene. Of the nine mutations, six also showed changes in amino acids. When comparing our isolate with the one isolated from the Korea Centers for Disease Control and Prevention (BetaCoV. SARS-CoV-2 reference genome was obtained from the GenBank database (NC_045512.2). Genomes alignment was performed using Muscle and Jalview software. We, then, calculated the Case Fatality Rate of SARS-CoV-2 in the Countries selected. Results. In this study we analyse how different lockdown strategies and PCR testing capability adopted by Italy, France, Germany, Spain, Sweden, UK and USA have. The primer/probe set (N2) amplified the nucleocapsid (N) gene of SARS-CoV-2 (NC_045512.2). The reaction mixture was 5 μL 4× TaqMan Fast Virus 1-Step Master Mix (Thermo Fisher Scientific), 1.0 μL 10 μM forward primer (5′-AAATTTTGGGGACCAGGAAC-3′), 1.4 μL 10 μM reverse primer (5′-TGGCAGCTGTGTAGGTCAAC-3′), 0.8 μL 5 μM probe (5. Sequence reference: NC_045512.2 Codon optimized and R683/5A mutations; ORF size: 3765 bp Sequencing primers: - Forward HTLV 5'UTR: TGCTTGCTCAACTCTACGTC - Reverse SV40 pAn: AACTTGTTTATTGCAGCTT Quality Control: - Plasmid construct is confirmed by restriction analysis and full‑length open reading frame (ORF) sequencing. - After purification by ion-exchange chromatography, predominant. Description. The NCBI Gene track for the 13 Jan 2020 SARS-CoV-2 virus/GCF_009858895.2 genome assembly is constructed from the NCBI nuccore entry for NC_045512.2 https.
the genbank dataset of the SARS-CoV-2 reference sequence NC_045512, which we suggest to use as the reference sequence in any of your analyses. The Illumina-sequenced data subfolder contains single-end and paired-end datasets, which can be distinguished by their names: the names of single-end datasets consist only of an SRR or ERR identifier followed by a file type suffix, while paired-end data. Biotechnology1 (NCBI) database, with ID NC_045512. The genome of SARS-CoV-2 is a 29,903 bp single-stranded RNA (ss-RNA) coronavirus. It has now been shown that the virus causing COVID-19 is a SARS-like coronavirus that had previously been reported in bats in China. 3. TISSUE DISTRIBUTION OF ACE2 IN HUMAN ORGANS AND TISSUES In order to discover the neurovirulence of SARS-CoV-2 and relate it to. NCBI is an active partner of the Vertebrate Genomes Project (VGP), who recently published a series of papers on the initial results of their efforts to sequence all 70,000 vertebrate species. See the VGP press release for more details. To date, this project has submitted over 130 diploid chromosome-level assemblies to NCBI's GenBank and the European Nucleotide Archive The Proteins of Severe Acute Respiratory Syndrome Coronavirus-2 (SARS CoV-2 or n-COV19), the 199 1 3 2 D: Prot SARS CV‑2 SARSCoV-2(NC_045512.2)hasatotalof11geneswit Packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication (By similarity)
Materials. All of the analyses and prediction were based on the NCBI Reference Sequence: NC_045512.2. On the basis of previous research of our group, 3624 genome sequences from GISAID (up to April 6th, 2020) were downloaded to construct a dataset for conservation analysis (Additional file 4: Table S1) .The structure of S protein(PDB ID: 6 × 6p) was downloaded from RCSB PDB , which has a. Human SARS-CoV-2 (NC_045512.2) Bat SARS-like CoV CoVZC45 (MG772933.1) Bat SARS-like CoV CoVZXC21 (MG772934.1) Bat CoV RaTG13 (MN996532.1) Human SARS CoV (NC_004718.3) Bat SARS-related CoV F46 (KU973692.1) Bat SARS-like CoV YNLF_31C (KP886808.1) Bat CoV LYRa11 (KF569996.1) Bat SARS CoV HKU3-7 (GQ153542.1) Bat SARS-like CoV RsSHC014 (KC881005.1) Bat CoV HuB2013 (KJ473814.1) Bat SARS-like CoV. By using the initially reported sequence MN908947.3, a BLAST search of the NCBI database revealed 6 inputs for the virus with essentially identical sequences (accession NC_045512.2, MN908947.3, MN975262.1, MN985325.1, MN988713.1, and MN938384.1)
genome (GenBank accession no. NC_045512.2)(25). Sequences were aligned with MAFFT v7.453 (26), and a phylogenetic tree was constructed using FastTree (version 2.1.1) (27) with the 5= and 3= untrans-lated regions (UTRs) masked. The resulting phylogenetic tree was visualized in R (version 3.6.1) using the ggtree package (28). Strains most closely related to the major outbreak clade were. The devastating effects of the recent global pandemic (termed COVID-19 for coronavirus disease 2019) caused by the severe acute respiratory syndrome coronavirus-2 (SARS CoV-2) are paramount with new cases and deaths growing at an exponential rate. In order to provide a better understanding of SARS CoV-2, this article will review the proteins found in the SARS CoV-2 that caused this. When running the dev nf-core/viralrecon v1.2.0dev (224865c), I noticed the multiQC report does not have information for Kraken2 % non-host reads even though the kraken2 reports are generated correctly. There are NAs instead of actual per.. NC_045512_N.2-102 67: Back To Top. More Information. Set Membership: Amplicon length less than or equal to 70 Viruses Inventoried Back To Top. Related Products. TaqMan ® Fast Advanced Master Mix; SuperScript ® VILO ™ cDNA Synthesis Kit ; Brands ; Shopping Tools. The NxSeq™ SARS-CoV-2 Whole Genome Library Kit includes primer pairs designed against the Wuhan-Hu-1 strain (NC_045512.2), where the sets are optimised to produce high on-target rates and consistent amplicon yields to maximise multiplexing, streamline analysis and keep costs low. Go from cDNA to final library in just 2 hours! Examine our rapid library preparation Drive your sequencing.